FIG. 6.

AR-Tubb3 ARE Binding. A) EMSA using full-size AR constructs were performed with wild-type ARE1 and ARE4 radiolabeled probes, as described in Materials and Methods. Anti-AR antibody was added where indicated to demonstrate specificity of AR binding. Arrow, DNA-receptor complex; arrowhead, supershifted complex. B) ChIP analysis of adult mouse testes chromatin. Fold enrichment is expressed as the ratio of recovered DNA in the specific antibody sample over the nonspecific antibody sample (1-fold enrichment = no enrichment) as explained in Materials and Methods. Values are given as mean ± SEM for five independent experiments for the Tubb3 ARE region (ARE1) and a negative control region located 4.2 kb upstream of the Tubb3 ARE1 (Ctrl).