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. 2011 May 12;85(2):315–326. doi: 10.1095/biolreprod.111.091082

FIG. 1.

FIG. 1

A) Schematic representation of the pAdT-CMR vector construct shows the sites for tandem insertion of shRNAs at positions 1, 2, and 3. B) Specific TRPC mRNA knockdowns produced by adenovirus vector expressing tandem TRPC1 (TC1sh), TRPC4 (TC4sh), and TRPC1+TRPC4 (TC1+4sh) shRNAs in UtSMC cells are shown. Left: TRPC1 mRNA knockdown was achieved by tandem expression of TC1sh and TC1+4sh but not TC4sh, compared to effects of control shRNA (Rsh). Right: Tandem expression of TC4sh and TC1+4sh, but not TC1sh, induced a TRPC4 mRNA knockdown when compared to Rsh. Data represent means ± SEM (n = 4).