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. 2015 Jun 24;6:7450. doi: 10.1038/ncomms8450

Figure 2. Deletion of Pak1 reduces atherosclerotic plaque progression.

Figure 2

(a) Representative en face staining of aortas from ApoE−/− and ApoE−/−:Pak1−/− mice fed with WD for 16 weeks are shown and the plaque area is presented as lesion % (that is, % of whole aorta) in the graph. Each symbol represents one animal and the horizontal bar indicates the mean value. (b) Representative Oil red O staining of the aortic root sections of the mice described in a is shown and the graph represents the quantification of the area positive for lipid staining. (c) Aortic root sections of the mice described in a stained for Mac3 are shown and the graph represents the quantification of the area positive for macrophages. Aortic root cryosections of the mice described in a were analysed by double immunofluorescence staining for CD36 in combination with SMCα-actin or Mac3 (d,e), or Filipin in combination with SMCα-actin or Mac3 (f,g). Bar graph represents the quantification of aortic root sections for CD36 and Filipin staining (n=6). Data were presented as mean±s.d. and assessed by Student's t-test. *P<0.05 versus ApoE−/− mice. Scale bars, 1 mm (a), 100 μm (b,c) and 50 μm (d–g).