Table 3.

Long-Range Couplings Observed in the HMBC Spectra of the New Premithramycin (11, 12) and Mithramycin Derivatives (13, 14) (Most Important Couplings Are Highlighted in Bold)

H-position	C-position
	11	12	13	14
2			1,3,1′,1C	3,1′,1C
3				4
4	1,4-OCH3,4a,5
α			4a	4a,10
β			2,3,4a,9a,10	2,4a,9a,10
4-OCH3	4	4
4a
5			6,7,8,8a,9,9a,10	6,7,10
α	4a,5a
β
6	5,6a,7,10a,11a	5,7,11a
7	6,8,9,10a	6,8,9,10a
7-CH3			5,6,7,8,8a,9a	6,7,8,10a
9		7,8,10,10a
9-CH3	8,9,10
10			4,5,8a,9a,10a	4,5,8,8a,9,9a,10a
14	2,13	13
1′			2,3,4,1′-OCH3,2′	3,4,1′-OCH3,2′
1′-OCH3			1′,3′	2,1′
3′			2′,4′,5′	2′
4′			2′,5′
5′			2′,3′,4′	3′,4′
1A	12a		6	6,2A
2Aa	1A,3A	1A,3A	1A,3A	1A,3A
2Ae	3A,4A	3A,4A	1A,3A,4A	1A,3A,4A
3A	1B	1B	2A,4A,1B	1B
4A			3A,5A,6A	3A,5A
5A	1A,6A	1A,6A	1A,3A,4A,6A	1A,4A
6A	4A,5A	4A,5A	4A,5A	5A
1B	3A	3A	3A	3A,2B
2Ba	1B,3B	1B,3B	1B,3B,4B	1B,3B
2Be			1B,3B,4B	1B,3B,4B
3B			2B,4B,5B	4B
4B	5B	5B	3B,5B,6B	3B,5B
5B	4B,6B	4B,6B	1B,3B,4B,6B	1B,3B,4B
6B	5B	5B	3B,4B,5B	2B,5B
1C				2,2C
2Ca			1C,3C	1C,3C
2Ce			1C,3C,4C	1C,3C,4C
3C			2C,1D	4C,1D
4C				3C,5C
5C			1C,3C,4C,6C	1C,4C
6C			4C,5C	2C,4C,5C
1D			3C	3C
2Da			1D,3D	1D,3D
2De			4D
3D			2D,4D
4D			2D,3D,5D	2D,3D
5D			1D,4D,6D	3D,4D
6D			4D,5D
1E				2E
2Ea				1E
2Ee				1E,4E
3E-CH3				1E,2E,3E,4E
5E				1E,4E
6E				4E,5E