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. Author manuscript; available in PMC: 2015 Dec 4.
Published in final edited form as: Nature. 2015 Mar 11;522(7554):89–93. doi: 10.1038/nature14319

Figure 3. Disruption of Dnmt3a in the brain leads to length-dependent up-regulation of genes containing high levels of mCA.

Figure 3

a, Summary of genome-wide bisulfite-sequencing analysis of mCN (where N = G, A, T, or C) in control and Dnmt3a cKO cerebella (n=2 per genotype). Dashed line represents mean background non-conversion rate of the bisulfite-seq assay (see Methods). b, Mean fold-change in gene expression versus gene-body mCA for MeCP2 KO (left) or Dnmt3a cKO (right) cerebella. Long (top 25%, >60kb) and short (bottom 25%, <14.9kb) genes were binned according to gene-body mCA/CA levels. Lines represent mean fold-change in expression for each bin (200 gene bins, 40 gene step), and the ribbon is S.E.M. of genes within each bin. ***, p < 0.005; two-tailed t-test, Bonferroni correction. Error bars represent S.E.M.