Figure 1. 1,25(OH)2D3 inhibits GC cell proliferation and promote cell apoptosis in vitro.

(A–B) SGC-7901 and AGS cells were treated with various doses of 1,25(OH)₂D₃ and the effects were determined by MTT assay after 24, 48 and 72 hours. (C–D) SGC-7901 and AGS cells were treated with 500 nM 1,25(OH)₂D₃ for 48 hours and cell cycle distribution was analyzed by flow cytometry. Histogram indicated the percentage of cells in G1, S and G2 cell-cycle phases. (E–F) SGC-7901 and AGS cells were treated with 500 nM 1,25(OH)₂D₃ for 48 hours, apoptosis was determined by Annexin V staining and flow cytometry. The experiments have been repeated 3 times, representative results of 3 independent experiments were shown. Data shown are mean values. (*P < 0.05; **P < 0.01; ***p < 0.001.)