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. 2015 Feb 18;6(10):7675–7685. doi: 10.18632/oncotarget.3048

Figure 6. miR-145 inhibits cell proliferation through E2F3 dependent cell cycle regulation.

Figure 6

(A) qRT–PCR were performed to determine the expression level of E2F3 after transfection of si-E2F3 (*P < 0.05, Student's t-test). (B) MTT assay was performed to determine the growth of SGC-7901 cells treated with si-E2F3. Data are reported as mean ± s.d. for three independent experiments. (C) Cell cycle determined in SGC-7901 cells 48 h after transfection of si-E2F3 by propidium-iodide staining flow cytometry. Histogram indicated the percentage of cells in G1, S and G2 cell-cycle phases. (D) Protein expression analysis for E2F3 dependent cell cycle regulation proteins in miR-145 over-expression construct, with empty vector orsi-E2F3, si-control-transfected SGC-7901 cells by western blotting.