Figure 4. RNASET2 protein re-localizes to P-bodies in response to chemically-induced hypoxia.

A) SKOV3 cells were seeded on coverslips, treated with 200 μM CoCl₂ for 24 hours and then processed for double IIF. No co-localization signal between RNASET2 and DCP-1A was detected in untreated SKOV3 cells, while a partial overlap (white arrowheads) was detected following treatment. Scale bar: 10 μm. B) A partial re-localization of RNASET2 protein to P-bodies (white arrowheads) was also detected in OVCAR3 cells following treatment. Confocal microscopy images. Scale bar: 25 μm. DCP-1A: de-capping enzyme 1A (PBs marker).