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. 2015 Mar 8;6(10):7851–7865. doi: 10.18632/oncotarget.3490

Figure 5. RNASET2 protein affects the structural organization of the actin cytoskeleton and is readily internalized by OVCAR3 cells.

Figure 5

A) OVCAR3 cell clones were seeded on coverslips and stained with TRITC-conjugated Phalloidin. A complex network of actin filaments is evident in control OVCAR3 cells, while the actin cytoskeleton appears disrupted in RNASET2-silenced cells. After addition of recombinant RNASET2, RNASET2-silenced OVCAR3 cells restored the original cytoskeletal pattern. Scale bar: 10 μm. B, C) RNASET2-silenced OVCAR3 cells were seeded on coverslips, treated with recombinant RNASET2 protein and then processed for IIF. B) In unpermeabilized cells, RNASET2 localizes to the cell surface, while after cell permeabilization a cytoplasmic signal was detected, consistent with the internalization of the protein (white arrowheads). C) Co-staining RNASET2 protein with phalloidin-TRITC showed no evident colocalization signal between the F-actin and the internalized protein. Confocal microscopy images. Scale bar: 10 μm.