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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

Fig.1. Tetrandrine induces NB4 cell autophagy and differentiation and represses tumor growth in vivo.

Fig.1

Fig.1

NB4 cells were inoculated into mice to establish a tumor model, as indicated in the Materials and Methods. Mice bearing tumors were randomly placed into three groups (8 mice /group) and were treated daily with vehicle or tetrandrine (25 mg/kg or 50 mg/kg) for 20 days. Animal weight and tumor volume was measured daily. (A) Mean tumor volumes after treatment. Values represent the means ± SD.*p<0.05. (B) Tumor weight after 20 days of treatment are presented in a scatter plot; the bars represent the ±SD. *p<0.05. (C) The mean values of the mouse body weights. (D) Western blot analysis of tissue lysates isolated from 25 (Tet 25 mg/kg) or 50 mg/kg (Tet 50 mg/kg) tetrandrine-treated or vehicle-treated NB4 cell xenografts. 4 mice in each group have been defined as “1#” to “4#”, respectively. (E) Notch1 (both full length and NICD proteins) levels and CD14 were evaluated by immunohistochemistry in tumor tissues derived from the 50 mg/kg tetrandrine-treated and the control mouse models. Magnification: × 400. (F) MDA level of tumor tissue proteins exacted from NB4 tumor xenografts. *p<0.05.