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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

Fig.2. Tetrandrine induced autophagy and differentiation in M5 type patient primary leukemia cells.

Fig.2

M5 leukemia is one subtype of acute myeloid leukemia (AML). (A) Western blot analysis of LC3 protein levels. M5 type patient cells were treated with 2 μM tetrandrine (Tet) for 24 hours. (B) Acridine orange staining assay analysis of autophagy in M5 type patient cells. Cells were treated with 2 μM tetrandrine (Tet) for 24 hours. Error bars represent the mean ±SD. **p <0.01. (C) Flow cytometry analysis of CD11b and CD14 expression. M5 type patient cells were treated with 2 μM tetrandrine (Tet) for 4 days.