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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

Fig.3. Tetrandrine (2 μM) inhibits acute promyelocytic leukemia NB4 cell proliferation.

Fig.3

Data are representative of values from at least three independent experiments. (A) NB4 cells were treated with 0 (DMSO), 0.5, 1, 1.5, 2, 2.5, or 3 μM tetrandrine for 24, 48 and 72 hours. The results show anti-proliferation effects (B) but minimal effects on cell viability. Data are represented as mean ± SD. (C) NB4 cells were treated with 0 (DMSO), 1, 2, or 3 μM tetrandrine (Tet) for 48 hours. Apoptosis was determined by annexin V-FITC/PI staining for 15 min at room temperature. (D) Flow cytometry analysis of cell cycle in NB4 cells after 2 μM tetrandrine (Tet) treatment for 48 h. (E) Western blot analysis of the cell cycle-related proteins p27kip1 and p21 in NB4 cells showed dose-dependent and (F) time-dependent increases after tetrandrine (Tet) treatment.