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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

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Copyright: © 2015 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig.5 — Oxidant dimethylsulfoxide (DMSO) was used as a negative control (Con), and All-trans retinoic acid (RA) treatment was used as a positive control. (A) Cell morphology was observed under a microscope with or without Wright–Giemsa staining after 4 days of 2 μM tetrandrine (Tet) treatment. Data are represented as mean ± SD. **p <0.01. (B) CD11b and CD14 antigens expression was measured alone (C) or co-measured by flow cytometry after 4 days of tetrandrine (Tet) treatment. (D) The respective cell surface expression of the CD11b and CD14 antigens, on NB4 cells after tetrandrine (Tet) treatment at the indicated concentrations and times was measured by flow cytometry. (E) NBT reduction assay analysis of differentiation. NB4 cells treated with 2 μM tetrandrine (Tet) for 24h-96h. Data are represented as mean ± SD. **p <0.01. (F) The relative levels of MMP9 expression were measured by RT-PCR after 2 μM tetrandrine (Tet) treatment for 24h-96h. Data are represented as mean ±SD. *p <0.05.

Fig.5 — Oxidant dimethylsulfoxide (DMSO) was used as a negative control (Con), and All-trans retinoic acid (RA) treatment was used as a positive control. (A) Cell morphology was observed under a microscope with or without Wright–Giemsa staining after 4 days of 2 μM tetrandrine (Tet) treatment. Data are represented as mean ± SD. **p <0.01. (B) CD11b and CD14 antigens expression was measured alone (C) or co-measured by flow cytometry after 4 days of tetrandrine (Tet) treatment. (D) The respective cell surface expression of the CD11b and CD14 antigens, on NB4 cells after tetrandrine (Tet) treatment at the indicated concentrations and times was measured by flow cytometry. (E) NBT reduction assay analysis of differentiation. NB4 cells treated with 2 μM tetrandrine (Tet) for 24h-96h. Data are represented as mean ± SD. **p <0.01. (F) The relative levels of MMP9 expression were measured by RT-PCR after 2 μM tetrandrine (Tet) treatment for 24h-96h. Data are represented as mean ±SD. *p <0.05.