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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

Fig.6. Early stage autophagy is related to tetrandrine-induced differentiation.

Fig.6

(A) Acridine orange staining assay analysis of autophagy. NB4 cells treated with Oxidant dimethylsulfoxide (Con), 2 μM tetrandrine (Tet), 1.5 mM 3-methyladenine (3-MA), and 2 μM tetrandrine (Tet) after a 1-hour pretreatment with 1.5 mM 3-MA (Tet+ 3-MA) for 10 hours. Error bars represent the mean ±SD. **p <0.01. (B) Western blot analysis of tetrandrine (Tet) induced LC3-II protein levels in the presence or absence of 1.5 mM 3-MA pretreatment for 10 hours. (C) CD14 was detected by flow cytometry of NB4 cells pretreated with 3-MA and incubated with tetrandrine (2 μM) for 4 days. (D) Western blot analysis of LC3-II and CTSD protein levels. Cells were treated with DMSO (Con), 2 μM tetrandrine (Tet), 15 mM chloroquine (CQ), and 2 μM tetrandrine (Tet) after a 1-hour pretreatment with 15 mM chloroquine (Tet+ CQ) for 24 hours. (E) CD14 was measured by flow cytometry of NB4 cells that were pretreated with 15 mM CQ and then treated with tetrandrine (2 μM) for 4 days.