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. 2015 Mar 10;6(10):7992–8006. doi: 10.18632/oncotarget.3505

Fig.8. Intracellular ROS generation is an early event of tetrandrine-induced cell autophagy and differentiation.

Fig.8

(A) Effects of tetrandrine (1, 2 μM) on intracellular ROS levels after 24 hours treatment. (B) NB4 cells were treated with DMSO (Con), 2 μM tetrandrine (Tet), 10 mM N-acetyl cysteine (NAC) or 0.2 mM Tiron, and 2 μM tetrandrine (Tet) after a 1-hour pretreatment with 10 mM NAC or 0.2 mM Tiron (Tet+ NAC or Tiron) for 24 hours. Intracellular ROS levels were measured by flow cytometry and (C) autophagy was detected with acridine orange staining assays. Error bars represent the mean ±SD. **p <0.01. (D) Western blot analysis of LC3, NICD and HES1 levels in cells after 1-hour pretreated with NAC for 24 hours. (E) Flow cytometry analysis of CD14 antigens expression on the surface of NB4 cells after 4days treatment.