Figure 5. Suppression of bubbling by NOS2 inhibitor Nω-LAME.

(A) COS7 cells were pretreated with Nω-LAME (0.125–4 mM) for 30 min, followed by exposure to UV irradiation (480 mJ/cm²) and cold shock (4°C) for 30 to 180 min. (B) Induction of NOS2 expression by UV/cold shock was shown in 30 min. The levels of intracellular NFκB (p65) and α-tubulin remained largely unchanged. A representative data is shown from 2 experiments. (C–D) By immunofluorescence microscopy, UV/cold shock together significantly induced the expression of NOS2 in COS7 cells (n = 10; mean ± standard deviation; Student's t-test).