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. 2015 Mar 10;6(10):8132–8143. doi: 10.18632/oncotarget.3517

Figure 2. siRNA-K9.3 reduces cell viability of HCT15 and A549 cells.

Figure 2

A. Decreased expression of KV9.3 mRNA by KV9.3 siRNA treatment in HCT15 and A549 cells. The cells were harvested 48 h after KV9.3 siRNA or negative control RNA transfection. Real-time PCR was performed for 45 cycles to quantify the KV9.3 mRNA level. Each bar represents the mean ± S.E.M. (n=4, ***P < 0.001 by the Student's t-test versus negative control RNA treated group, NC: negative control) B. KV9.3 knockdown decreases cell viability of HCT15 and A549 cells. Cell viability was measured by MTT assay 72 h after KV9.3 siRNA transfection. Each bar represents the mean ± S.E.M. (n=9, three independent experiments, ***P < 0.001 by the Student's t-test versus negative control RNA treated group).