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. 2014 Dec 16;6(10):8155–8166. doi: 10.18632/oncotarget.2692

Figure 5. Gamma H2AX staining.

Figure 5

Fluorescence photomicrographs show the merging of cells stained with anti-H2AX (green), anti Ki-67 (red) and Hoechst 33342 (blue). A representative microscopic field for each treatment is shown. – Graph shows the degree of H2AX phosphorylation. This was evaluated by counting the number of gamma-H2AX immunofluorescent foci per cell. Foci number was determined for 200 cells. Each dot represents an individual cell. Black bars indicate mean value for each category (n = 3, *p < 0.05, **p < 0.01).