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. 2015 Mar 13;6(10):8286–8299. doi: 10.18632/oncotarget.3221

Figure 6. miR-93 activates PI3K/Akt signaling.

Figure 6

(A) Relative Akt activity in the indicated cells, determined by K-LISA Akt Activity assay. (B) Western blotting analysis of phosphorylated Akt (p-Akt) (Ser 473), total Akt, Cyclin D1 and p27Kip1 protein levels in indicated cells. α-Tubulin was used as a loading control. (C) The mRNA expression level of Cyclin D1 and p27Kip1, determined by real-time PCR analysis. (D) Quantification of colonies formed determined by colony formation assay in indicated glioma cell lines, treated with Akt inhibitor (0.5 μM). (E) Quantification of colonies determined by anchorage-independent growth ability assay in indicated glioma cell lines, treated with Akt inhibitor (0.5 μM). (F) Quantification of BrdUrd incorporating-cells in indicated glioma cell lines, treated with Akt inhibitor (0.5 μM). (G) The mRNA expression level of Cyclin D1 and p27Kip1, determined by real-time PCR analysis, in indicated glioma cell lines, treated with Akt inhibitor (0.5 μM). Experiments were repeated at least 3 times with similar results, and error bars represent ± SD. *P < 0.05.