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. 2015 Mar 21;6(10):8353–8365. doi: 10.18632/oncotarget.3229

Figure 2. HOTAIR inhibited NLK transcription in vitro.

Figure 2

(A) Lenti-HOTAIR si treatment induced NLK expression in U87 and U87vIII GBM cell lines after 48 h. (B) U87 and U87vIII GBM cell lines were incubated with 5 μmol/L DZNEP or 2PCPA for 48 h, and DZNEP treatment increased the level of NLK. (C) Astrocytoma cells were treated with Lenti-HOTAIR 3′ or 5′ domain for 48 h, and treatment with Lenti-HOTAIR 5′ domain inhibited NLK expression. GAPDH was used as a loading control. (D) and (E) The interaction of H3K27me3 and NLK-encoding gene regulatory elements (an approximately 5 kb region upstream from the transcriptional start site) was determined by CHIP assay.