Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Exp Hematol. 2015 Apr 16;43(7):578–585. doi: 10.1016/j.exphem.2015.04.001

Figure 1. CD201 and CD27 are expressed in both B6 and NOD bone marrow, and stain a subset of the Kit + progenitor population.

Figure 1

A. NOD and B6 bone marrow cells were stained for Kit, Sca-1, CD27 and CD201. Each plot shows pre-gated live, lin− cells, and each column of 3 plots is from an individual stained sample. Both the D7 and E13.1 clones of Sca-1 stain a distinct progenitor population in B6 BM, but not in NOD BM (top panels). In contrast, a distinct CD27+CD201+ population is present in both B6 and NOD BM (middle panels). The CD27+CD201+ population is overlaid onto the Kit vs. Sca-1 plot (bottom panels). B. Plots of CD201 versus Sca-1 staining in B6 and NOD BM progenitors show that the CD201+ progenitor population is high for Sca-1 in B6 and low for Sca-1 in NOD BM. Plots are pre-gated on live, lin−Kit+ cells. C. Comparison of mean fluorescence of Sca-1 staining in the lin−CD27+CD201+ BM populations from B6 and NOD mice with both the D7 and E13 clones.