Figure 2. The lin⁻CD27⁺CD201⁺ population contains the vast majority of HSC in NOD BM.

A. Lin⁻ NOD BM was sorted into two fractions, a CD27⁺CD201⁺ population and the “not” CD27⁺CD201⁺ population, which contains all the remaining cells. These populations were co-transplanted from mRaspberry and non-fluorescent NOD donors into lethally-irradiated non-fluorescent NOD recipients. B. Three months post-transplantation, peripheral blood donor chimerism was measured. FACs plots show chimerism of one representative recipient of mRasp⁺CD27⁺CD201⁺ BM. C. Peripheral blood chimerism at 3 months post-transplantation within each transplanted mouse (*p=0.0023, unpaired student's t-test). Peripheral blood of an untransplanted mRasp mouse (untranspl.), and one mouse receiving 0.5 BM equivalents of lin⁻ mRasp BM cells (lin⁻ control) are shown as controls. D. CD27⁺CD201⁺ cells self-renew: three month chimerism of donor derived lin⁻CD27⁺CD201⁺ cells in the BM of mice transplanted with mRasp⁺CD27⁺CD201⁺ (n=4) or mRasp⁺ “not” CD27⁺CD201⁺ BM progenitors (n=4) (*p=0.0356, unpaired student's t-test).