Figure 3. CD201 and CD27 stain progenitors independently of variation in Sca-1 expression across strains.

A. The Kit⁺Sca-1⁺ stains are shown for 8 strains of mice (left panels). While the KLS population is only apparent in some strains, all tested mouse strains exhibit a distinct CD27⁺CD201⁺ population (middle panels), which completely or partially overlaps with the Kit⁺Sca-1⁺ population in mice that express Sca-1 (right panels). Plots are pre-gated on live, lin⁻ BM. B. CD27⁺CD201⁺ progenitors were transplanted into lethally-irradiated Rag^−/−GFP⁺ recipients and the percentage of donor-derived GFP⁻ granulocytes (CD45⁺CD3⁻CD19-Gr1⁺Mac1⁺) was assessed by flow cytometry of splenocytes at 8 weeks post-transplantation. C. Bone marrow was assessed for donor-derived GFP⁻ HSC and progenitor chimerism (lin-Kit⁺CD27⁺CD201⁺⁾ at 8 weeks post-transplantation by flow cytometry. In all strains, sorted CD27⁺CD201⁺ BM cells resulted in substantial HSC and progenitor chimerism. In both B and C, Rag^−/−GFP⁺ whole BM was co-transplanted to ensure survival.