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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Exp Hematol. 2015 Apr 16;43(7):578–585. doi: 10.1016/j.exphem.2015.04.001

Figure 4. CD27 and CD201 identify progenitors in a post-irradiation setting of altered Kit and Sca-1 expression.

Figure 4

A and B. Hematopoietic progenitors do not maintain expression of Kit 48 hours after sublethal 6 Gray (A) and 3 Gray (B) irradiation in B6 mice (n=2) as compared to a non-irradiated control (left panels). Within the same stained samples CD27 and CD201 levels are maintained and identify a clear progenitor population (second panels). This CD27+CD201+ progenitor population exhibits increased Sca-1 and decreased Kit expression (third panels). The CD27+CD201+ progenitor population expresses high levels of CD150, a hematopoietic stem and progenitor marker (fourth panels). All plots are pre-gated on live, lin− BM. C. CD27+CD201+GFP+ progenitors were isolated from the BM of irradiated mice 48 hours post irradiation (3 Gray) and transplanted competitively with 250,000 WBM from non-irradiated B6 mice (n=5). Peripheral blood was assessed for donor granulocyte, T cell and B cell chimerism at 8 weeks post transplant.