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Determination of malonyl CoA as the preferred substrate of the discrete AT (LnmG): (A) time-course assays of ATself-acylation with malonyl CoA (∎), methylmalonyl CoA (◆), butyryl CoA (▴), propionyl CoA (★), or acetyl CoA (◯); and (B) assays with a fixed amount of AT enzyme (2 μM of LnmG) and variable concentrations of malonyl CoA (∎) or methylmalonyl CoA (◆).
