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. Author manuscript; available in PMC: 2015 Jun 25.
Published in final edited form as: Methods Enzymol. 2009;459:165–186. doi: 10.1016/S0076-6879(09)04608-4

Table 8.2.

ESI-MS analysis of apo-, holo-, and malonyl-S-ACPs of the LnmI and LnmJ PKSs

apo-ACP [M + H]+
 holo-ACP [M + H]+
 Malonyl-S-ACP [M + H]+
ACPsa Calculated Found Calculated Found Calculated Found
LnmI-ACP3 11,702 11,700 12,042 12,040 12,128 12,126
LnmJ-ACP4 12,245 12,241 12,585 12,582 12,671 12,669
LnmJ-ACP5 12,520 12,517 12,860 12,857 12,946 12,943
LnmJ-ACP6-1 12,209 12,206 12,549 12,546 12,635 12,632
LnmJ-ACP6-2 12,151 12,147 12,491 12,486 12,577 12,572
LnmJ-ACP7 12,322 12,318 12,662 12,665 12,748 12,751
LnmJ-ACP8 12,090 12,087 12,430 12,427 12,516 12,512
a

The numbers after ACPs refer to the LNM PKS modules from which they are derived, with 6-1 and 6-2 to indicate the first and second ACPs, respectively, for LNM PKS module 6.