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Three-dimensional reconstruction of images of cultured primary AECs on a Transwell insert with (c, d) or without (a, b) co-culture of BMSCs in the bottom well (separated co-culture) on days 7 (a, c) and 21 (b, d). Red staining indicates expression of RAGE, yellow staining indicates ZO-1 expression, and green staining indicates expression of SP-D
