Fig. 3.

Serum antibody titers induced in Balb/C mice immunized with the synthetic peptides. Seven groups (pr1, pr3, pr4, pr9, pr10, PM10, and PBS), ten mice for every group, were immunized three times with 100 μg of immunogens. All peptides were coupled to KLH before immunization. PM10 and PBS were used as controls. The antisera were collected on week 2, 4 and 6 via tail vein, and the antibody titer of mice sera was detected by ELISA. The antibody titer of anti-pr4 sera was remarkably higher than that of the antisera from groups of pr1, pr3, pr9, pr10, PM10, and PBS at any time point. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD); *p < 0.05 vs other groups (pr1, pr3, pr9, pr10, PM10, and PBS)