Fig. 5.

Characterization of epitope peptide pr4. a ELISA reactivities of synthetic peptide pr4 with immunized mice sera. Synthetic peptide pr4 could react with anti-DENV1-4 mice sera but not with anti-JEV mice sera and normal mice sera (NMS). b Competitive inhibition of prM protein binding to anti-prM pAb by synthetic peptide pr4. Competitive ELISA was performed using pr4 as competitor of prM protein. The reaction activity of anti-prM pAb with prM protein was inhibited markedly by pr4 in a concentration-dependent manner. c, d ELISA reactivities of synthetic peptide pr4 with sera from 20 DENV2-infected patients (c) and 20 healthy adults (d). pr4 was able to react with sera from 18 patients infected with DENV2 but not with sera from healthy adults. PM10 was used as control. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD). If there is no error bar, it is not that no variations among three independent experiments but that the variations are too small to show in the figure