. Author manuscript; available in PMC: 2016 Mar 1.

Published in final edited form as: Bioanalysis. 2015 May;7(8):1037–1058. doi: 10.4155/bio.15.35

Table 3.

Published LC–MS/MS assays for quantification of immunosuppressive drugs in fingerprick samples.

Drug/reference	Subjects	Blood sample volume	Extraction method	Chromatographic conditions	Precursor ion (m/z) > product ion (m/z)	Ref.
CsA	HTRs and LgTRs (n = 65); RTRs (n = 33)	10 μl	Pretreatment with 0.1 mmol zinc sulfate solution and LLE with CAN	Gradient: H₂O (A), MeOH (B), both contain 2 mmol ammonium acetate/0.1 FA	[M + NH4]+	[11–13]
			IV: 5 μl	Online SFE: SecurityGuard C18 cartridge (Phenomenex)	CsA 1220 > 1203
				Run time: 2.5 min	IS: ASC/CsD
				LLOQ: 10 μg/l

TAC	RTRs (n = 33); RTRs & pancreas (n = 2); and RTRs & HTRs (n = 1) children	10 μl	Pretreatment with 0.1 mmol zinc sulfate solution and LLE with CAN	Gradient: H₂O (A), MeOH (B), both containing 2 mmol ammonium acetate/0.1 FA	[M + NH4]+	[14]
			IV: 10 μl	Online SFE: SecurityGuard C18 cartridge (Phenomenex)	TAC: 821 > 768
				Run time: NR	IS: ASC
				LLOQ: 0.5 μg/l

ACN: Acetonitrile; Ammonium adduct: [M+NH4]+; ASC: Ascomycin; CsA: Cyclosporine A; CsD: Cyclosporine D; FA: Formic acid; HTR: Heart transplant recipient; IV: Injection volume; LgTR: Lung transplant recipient; MeOH: Methanol; R: Recovery; RTR: Renal transplant recipient; TAC: Tacrolimus.