Table 4.
Tropomyosin 1 protein expression in breast cancer cell lines.
| Cell line | Antibody and TPM isoforms detected▲ | |||||||
|---|---|---|---|---|---|---|---|---|
| TM311Δ | TPM1E2a | CH1 | ||||||
| >40 kD | 40 kD | 38 kD | 36 kD | 34 kD | <34 kD | |||
| TPM2γ | TPM1β, κ | TPM2β, η | TPM1α, γ | TPM1δ, η | TPM2ε, ζ | TPM1β, κ, ξ | TPM1α, κ, μ, ξ | |
| TPM2α | ||||||||
| TPM2α, δ | TPM4δ, ε | TMP3α | TPM3α, δ, ε, θ, ι, κ, λ, μ, ν | |||||
| Breast cancer | ||||||||
| HCC 1143∗ | − | − | ++ | ++ | − | − | − | − |
| HCC 1187∗ | − | − | ++ | +++ | − | − | − | − |
| BT 474 | − | − | − | + | − | − | − | − |
| mDAMB 157 | − | − | + | ++++ | − | − | − | − |
| HCC 1806 | − | − | + | + | − | − | − | − |
| HCC 1419∗ | − | − | + | + | − | − | − | − |
| mDAMB 453 | − | − | ++ | ++ | − | − | − | − |
| mDAMB 468 | − | − | ++ | +++ | − | − | − | − |
| MCF7 | − | − | ++ | ++ | − | − | − | − |
|
| ||||||||
| MCF10A (normal breast) | − | − | +++ | ++ | − | − | − | − |
| HCC 1143 (BL)+ | − | − | − | − | − | − | − | − |
| HCC 1187 (BL)+ | − | − | − | − | − | − | − | − |
|
| ||||||||
| Adult heart | − | + | + | ++++ | − | − | + | ++++ |
| Fetal heart | − | + | + | ++++ | − | − | + | ++++ |
| Skeletal muscle | − | +++ | − | +++ | +++ | − | − | ++++ |
The symbols ∗ and + are as described in Table 1.
▲ = cell extracts were analyzed by western blot using the various primary antibodies indicated. Expression was qualitatively recorded as either negative (−) or plus (+) to 4 pluses (++++).
Δ = the assignations of various TPM protein isoforms to particular sizes on an SDS PAGE gel are as per Schevzov et al. [23].
TPM1λ, μ, ν, and ξ would be detected by TM311, but we do not know their sizes on SDS PAGE.