Table 4. Characteristics of immune sera from monkeys used for the inhibition of the hDARC/PvDBPII interaction.
| Monkey Code | Acute infection b | Anti-PvDBPII IgG, RI c | Percentage of inhibition |
|---|---|---|---|
| BL69 | Pos | 22 | 44% |
| BL64 | Pos | 18 | 40% |
| BL10 a | Pos | 12 | 36% |
| BL34 | Neg | 22 | 43% |
| BL37 | Neg | 14 | 94% |
| BL40 | Neg | 7 | 10% |
| BL41 | Neg | 6 | 0 |
| BL44 | Neg | 1 | 0 |
aBL10 was the only symptomatic animal [24]
bPeripheral blood infection was detected using conventional microscopy, and P. vivax/P. simium identification was confirmed using amplification of the P. vivax 18SSU rRNA gene with two different PCR-based protocols (Nested and Real-time) [24]
cAnti-PvDBPII IgG antibodies were detected by ELISA using recombinant PvDBP (aa 132–771)[24], and the results are expressed as Reactivity Index (RI), which corresponds to OD492nm values of test samples divided by the cut-off value.
Values of RI > 1 were considered positive.