Fig 5. ProR-dependent, FKH-independent regulation of IL-2 upon activation.

(A) Activation-induced expression of IL-2 in Foxp3 or Foxp3 mutant transduced cells measured by flow cytometry. Transduced cells were rested and re-activated with cell stimulation cocktail (PMA and ionomycin with protein transport inhibitor) before staining. Data are representative of three independent experiments. (B) Expression of Il2 mRNA in Foxp3 or Foxp3 mutant transduced cells at resting or re-activation state was determined by quantitative RT-PCR relative to the expression of CD3ε. Circles indicate measurements from resting cells, square measurements from reactivated cells. Height of the bars indicates the mean of the two experiments.