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. 2015 Jun 24;10(6):e0129518. doi: 10.1371/journal.pone.0129518

Fig 4. Histological and immunomorphological analysis of post-natal eye growth.

Fig 4

Significantly increased axial length is first observed at P5 (A). The retinal lamination in P90 Lrp2 FoxG1.cre-KO mutant eyes is normal but all the retinal cell layers appear reduced; Nissl staining (B). The continuous reduction of the retinal thickness between P5 and P90 (C) is mainly due to the thinning of the inner nuclear (INL), outer nuclear (ONL) and inner plexiform (IPL) cell layers (D-F). The distribution of the typical retinal markers Brn3a, PKCalpha, PNA-Lectin (PNA-L) and Aquaporin 4 (Aq4) in the retinal ganglion, bipolar, photoreceptor and Müller cells respectively is similar in control (G) and mutant littermates (H). The number of PKCalpha, PNA-L and Aq4 positive cells appears decreased in the mutants. (I) PKCalpha immunoreactivity in bipolar cells shows a tight cluster of dendrites (arrow) in the OPL, an oval cell body (asterisk) in the INL, and a vertically directed axon, which expands into clusters of terminals (arrowheads) in the proximal portion of the IPL and GCL. (J) In the Lrp2 FoxG1.cre-KO mutant the PKCalpha positive bipolar cell has shrunk dendrites at the OPL-INL border (arrow) and a vertically directed axon, which expands abnormally in thick clusters (arrowheads) in the IPL and GCL. Two-way ANOVA post hoc Tukey test was used, **P<0.01, ***P<0.001, values are mean ± SEM of 10 animals per age and genotype. Scale bars: 50 μm in B, G, H; 70 μm in I, J.