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. 2015 Jul 1;38(7):1075–1083. doi: 10.5665/sleep.4816

Figure 8.

Figure 8

Starvation reduces Drosophila insulin-like peptide (dilp) level analyzed by quantitative polymerase chain reaction (qPCR). Profile of dilp level by qPCR in starved and unstarved flies. The 4-day-old wild-type (w118) flies were deprived for 24 h starting at Zeitbeger time (ZT) 0. The x-axis represents the ZT. RNA levels are normalized to the amount present in the flies at ZT0. Data were collected every 6 h. Error bars indicate the standard error of the mean from three independent replicates.