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. 2015 Apr 11;14:80. doi: 10.1186/s12943-015-0352-y

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© Chow et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The apoptotic effect of Raf265 on HT29 and HCT116 cells. A. HT29 cells and B. HCT116 cells were treated with 0–15 μM Raf265 and Annexin V assay was performed. Representing flow diagrams at 0 and 15 μM were shown at the upper panel and bar charts presenting the average percentage of annexin V positive cells after treatment were shown at the lower panel. C. HT29 cells and D. HCT116 cells were treated with 0–10 μM Raf265. Caspase 3, caspase 8 and caspase 9 activities were analyzed by flow cytometry. Data are presented as means ± SD from three independent experiments and statistical analysis was performed by one-way ANOVA. *p < 0.05 versus untreated control.