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. 2015 Apr 11;14:80. doi: 10.1186/s12943-015-0352-y

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© Chow et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inactivation of the Raf/MEK/ERK signaling pathway by Raf265 on HT29 and HCT116 cells. A. HT29 cells and B. HCT116 cells were treated with 0–10 μM Raf265 with or without the addition of EGF, or 10 μM U0126. Representing blots of the western blot analysis of the total expression and phosphorylation of MEK and ERK were shown. The expression level of β-actin was used as the loading control. At the lower panels, bar charts presenting the ratio of p.MEK vs. TL. MEK and p.ERK vs TL. ERK were shown. Data are presented as means ± SD from three independent experiments and statistical analysis was performed by one-way ANOVA. *p < 0.05 versus untreated control of the corresponding cell lines.