Fig. 7.

Complementation of the PA-deficient tt2 mutant phenotype by constitutively expressing Tc-MYBPA. a 7-day old seedlings of and DMACA stained seeds from Col-0, the tt2 mutant (SALK_005260) and three independent T2 transgenic lines of tt- 35S:Tc-MYBPA. The bar represents 1 mm. b RT-PCR analysis of Tc-MYBPA and AtUbiquitin transcripts in total RNA from the young seedlings shown in (a). PCR products from the Tc-MYBPA-pGEM plasmid were loaded on the last lane as a positive control for the Tc-MYBPA primer set and as a negative control for the AtUbiquitin primer set. C, PA levels in mature seeds of plants shown in (a). PA levels were determined by extraction and DMACA reaction using procyanidin B2 as a standard. All the data are presented as means ± SE, n = 3. **P < 0.01 versus tt2; ***P < 0.001 versus tt2. FW, fresh weight