Ninety six hours after MCAO, mononuclear cells were isolated from spleens of RPMI or IL-10-GFP+ B-cell recipient WT mice and analyzed for: A. Total cell count via hemocytometer. Values represent mean numbers (±SEM) of indicated cell subsets from 28-30 mice in each group, from at least 8 separate experiments; B. Comparison of CD11c+ dendritic cells, CD11b+ monocytes, CD19+ B-cells, CD4+ and CD8+ T-cell subpopulations. Values represent mean numbers (±SEM) of indicated cell subsets, gated on live leukocytes (by PI exclusion), from 8-12 mice of each group, from at least 3 separate experiments. Statistical analysis was performed with Student's t-test to compare between RPMI and IL-10-GFP+ B-cell recipient mice. Significant differences between sample means are indicated (*p≤0.05).