Figure 3. Transfer of IL-10-GFP⁺ B-cells to WT mice, 24 h post-MCAO, leads to reduced cerebral inflammatory cell infiltration.

Ninety six hours after MCAO, mononuclear cells were isolated from brains of RPMI and IL-10-GFP⁺ B-cell recipient WT mice and were analyzed for: A. Total cell count via hemocytometer. Values represent mean numbers (±SEM) of indicated cell subsets from 14-15 mice per group, from at least 5 separate experiments; and B. Percent frequencies of CD11b⁺CD45^high activated microglia (MG)/monocytes, CD11b⁺CD45^lo resting MG, CD4⁺ T-cells, CD8⁺ T-cells and CD19⁺ B-cells obtained from the non-ischemic (left) and ischemic (right) hemispheres of WT recipient mice by Flow cytometry. Values represent mean numbers (±SEM) of indicated cell subsets from 6 mice from WT mice transferred with medium or IL-10-GFP⁺ B-cells after 24 hour of MCAO, from at least 3 separate experiments. Statistical analysis was performed with ANOVA followed by Tukey's multiple comparison post-hoc test. Significant differences between sample means are indicated (*p ≤0.05 and **p ≤0.01) as compared to the ischemic right hemisphere of medium-treated WT recipient mice.