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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Metab Brain Dis. 2014 Dec 25;30(4):911–924. doi: 10.1007/s11011-014-9639-8

Figure 5. Treatment with IL-10+ B-cells, 24 h post-MCAO, leads to a significant increase in the CD8+ T regulatory cell population and its anti-inflammatory status in the ischemic hemisphere of the brain of recipient mice.

Figure 5

Brain leukocytes from ischemic hemispheres of RPMI and IL-10-GFP+ B-cell transferred WT recipient mice were harvested 96 h after MCAO and assessed for: A. Frequency of CD8+CD122+ T-cells. Data are representative of 5 independent experiments (mean ± SEM); and B. analyzed for IL-10-production on GFP− cells using IL-10 APC mAb for detection by Flow cytometry and for the expression of IL-10 (GFP−) on gated CD8+CD122+ T-cells; Data are representative of 5 independent experiments with spleens processed from 9-10 individual mice (mean ± SEM). Significant differences between the groups were determined using Student's t-test and are indicated (*p≤0.05 and **p≤0.01).