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. 2015 May 5;290(26):15909–15920. doi: 10.1074/jbc.M115.640136

FIGURE 4.

FIGURE 4.

Efficiency and selectivity of Actn4 knockdown in neurons. A, representative immunoblots of lysates from DIV 12 cortical neurons treated with control or Actn4 siRNAs and probed with antibodies against Actn4, Actn1, and Actn2 and control GAPDH. Arrowhead points to Actn4. B, quantification of Actn4 protein abundance in control or Actn4 siRNA-treated neurons. Mean ± S.E.; n = 3 independent experiments; **, p < 0.01. C, representative images of DIV 12 cortical neurons treated with control or Actn4 siRNAs and labeled with anti-Actn4 and anti-MAP2 antibodies. Scale bars = 35 μm. D, quantification of Actn4 fluorescence intensity in dendritic segments from images like those in C. Mean ± S.E.; control siRNA, n = 13 neurons; Actn4 siRNA, n = 12 neurons; ***, p < 0.001.