FIGURE 2.

Surface plasmon resonance analysis of the TNFSF members binding to vTNFRs. Four examples of the binding sensorgrams and fittings obtained for the determination of the kinetic constants of the TNFSF-vTNFR interactions: mTNF to CPXV CrmD (A), hTNF to CPXV CrmB (B), mLTα to ECTV CrmD (C), and hLTα to VARV CrmB (D). Binding and dissociation of several concentrations of TNFSF ligands at 30 μl/min were recorded and adjusted to a 1:1 Langmuir fitting (solid lines). The nanomolar concentration corresponding to each sensorgram is indicated. The arrowhead points the end of the injection.