TABLE 1.
Yeast strains and plasmids used in this study
| Strain/plasmids | Genotype/plasmid | Source |
|---|---|---|
| Strains | ||
| W303-1a | MATa ade2 his3 leu2 trp1 ura3 can1 | Lab stock |
| YIT2013 | W303-1a ski2Δ::kanMX | Ref. 6 |
| YIT2019 | W303-1a xrn1Δ::kanMX | Ref. 29 |
| YIT2020 | W303-1a xrn1Δ::kanMX dom34Δ::hygtMX | Ref. 4 |
| TYSC360 | W303-1a sen54Δ::HIS3, p314–54 CEN6-ARSH4 TRP1 SEN54 | Ref. 17 |
| TYSC361 | W303-1a sen54Δ::HIS3, p314–200 CEN6-ARSH4 TRP1 sen54-Δ200–232 | Ref. 17 |
| COSC05 | W303-1a sen2Δ::LEU2, pCOSC05 CEN6-ARSH4 TRP1 SEN2 | Ref. 17 |
| COSC06 | W303-1a sen2Δ::LEU2, pCOSC05 CEN6-ARSH4 TRP1 sen2–41 | Ref. 17 |
| YTS1612 | W303-1a sen54Δ::HIS3, p314–54 CEN6-ARSH4 TRP1 SEN54, ski2Δ::kanMX | This study |
| YTS1613 | W303-1a sen54Δ::HIS3, p314–200 CEN6-ARSH4 TRP1 sen54-Δ200–232, ski2Δ::kanMX | This study |
| YTS1614 | W303-1a sen54Δ::HIS3, p314–54 CEN6-ARSH4 TRP1 SEN54, xrn1Δ::kanMX | This study |
| YTS1615 | W303-1a sen54Δ::HIS3, p314–200 CEN6-ARSH4 TRP1 sen54-Δ200–232, xrn1Δ::kanMX | This study |
| YTS1616 | W303-1a sen2Δ::LEU2, | This study |
| pCOSC05 CEN6-ARSH4 TRP1 SEN2, ski2Δ::kanMX | ||
| YTS1617 | W303-1a sen2Δ::LEU2, pCOSC05 CEN6-ARSH4 TRP1 sen2–41, ski2Δ::kanMX | This study |
| YTS1638 | W303-1a sen2Δ::LEU2, pCOSC05 CEN6-ARSH4 TRP1 SEN2, xrn1Δ::kanMX | This study |
| YTS1639 | W303-1a sen2Δ::LEU2, pCOSC05 CEN6-ARSH4 TRP1 sen2–41, xrn1Δ::kanMX | This study |
| YTS1798 | W303-1a tom20Δ::natMX | This study |
| YTS1801 | W303-1a tom20Δ::natMX ski2Δ::kanMX | This study |
| YTS1803 | W303-1a puf3Δ::hygMX ski2Δ::kanMX | This study |
| YTS1806 | W303-1a tom20Δ::hygMX xrn1Δ::kanMX | This study |
| Plasmids | ||
| p416GPDp | URA3 CEN | Ref. 30 |
| p416GAL1p | URA3 CEN | Ref. 30 |
| p415GAL1p | LEU2 CEN | Ref. 30 |
| p414GAL1p | TRP1 CEN | Ref. 30 |
| p413GAL1p | HIS3 CEN | Ref. 30 |
| yCplac33 | URA3 CEN | Ref. 31 |
| pSA144 | p416GPDp-GFP-FLAG-HIS3 | Ref. 32 |
| pTYSC155 | CEN6-ARSH4 URA3 SEN54 | Ref. 17 |
| pTS152 | p416GAL1p-CBP1 | This study |
| pTS220 | p416GAL1p-CBP1-Δ652–678 | This study |
| pTS221 | p416GAL1p-CBP1-Δ679–702 | This study |
| pTS223 | p416GAL1p-CBP1-Δ703–726 | This study |
| pTS256 | p416GAL1p-CBP1-Δ727–738 | This study |
| pTS257 | p416GAL1p-CBP1-Δ739–762 | This study |
| pTS258 | p416GAL1p-CBP1-Δ763–789 | This study |
| pTS343 | p416GAL1p-CBP1-FS (+652–726) | This study |
| pTS344 | p416GAL1p-CBP1-FS (+727–789) | This study |
| pTS244 | p416GAL1p-CBP1-M1 | This study |
| pTS247 | p416GAL1p-CBP1-M2 | This study |
| pTS184 | p416GAL1p-stemloop-CBP1 | This study |
| pTS260 | p416GAL1p-stemloop-CBP1-ΔMTS | This study |
| pTS179 | p416GAL1p-CBP1-ΔMTS | This study |
| pTS225 | p416GAL1p-CBP1-ΔuORF | This study |
| pTS173 | p416GAL1p-CBP1-MMH2 | This study |
| pTS229 | p416GPDp-GFP-CBP1 (+643–738)-FLAG-HIS3 | This study |
| pTS231 | p416GPDp-CBP1 (+1–99)-GFP-FLAG-HIS3 | This study |
| pTS236 | p416GPDp-CBP1 (+1–99)-GFP-CBP1 (+643–738)-FLAG-HIS3 | This study |
| pTS324 | p416GPDp-CBP1 (+1–99)-GFP-CBP1 (FS 643–738)- FLAG-HIS3 | This study |
| pTS332 | p416GPDp-CBP1 (+1–99)-GFP-CBP1 (M1 643–738)- FLAG-HIS3 | This study |
| pTS318 | p415GAL1p-SEN2 | This study |
| pTS299 | p415GAL1p-His6-flag-SEN2 | This study |
| pTS300 | p415GAL1p-His6-flag-sen2 H297A | This study |
| pTS305 | p413GAL1p-SEN15 | This study |
| pTS314 | p414GAL1p-SEN54 | This study |
| pTS320 | p416GAL1p-SEN34 | This study |
| pTS351 | yCplac33TOM20p-TOM20 | This study |