FIGURE 1.

PDGFB transfection resulted in the induction of GD3 in p53^−/− astrocytes. After transfection of PDGFB, AKT, KRAS, or β-catenin in RCAS vector or vector alone into primary cultured astrocytes prepared from newborn mice, cells (3 × 10⁵) were detached by treatment with trypsin. After washing, cells were incubated with an anti-GD3 mAb or an anti-GD2 mAb for 1 h on ice. After washing, they were incubated with an FITC-labeled anti-mouse IgG antibody. Expression levels of GD3 and GD2 were analyzed by flow cytometry. The results of astrocytes from wild type Gtv-a mice were shown in the upper two panels, and those of astrocytes from p53^−/− Gtv-a mice were shown in the lower two panels. Green lines indicate the cells stained with either one of mAbs. Blue lines indicate the cells stained with a non-relevant antibody for control.