FIGURE 1.

Systemic effects of RSV and SRT are independent of skeletal muscle PGC-1α. CTRL and PGC-1α MKO mice were fed a HFD for 2 weeks and subsequently switched to a HFD containing either RSV (4 g/kg diet) for 4 weeks (RSV-S) or 13 weeks (RSV-L) or SRT (1 g/kg diet) for 4 weeks. Control groups were maintained on an unmodified HFD for a total of 6 weeks (RSV-S and SRT) or 15 weeks (RSV-L). A, body weight at the end of the treatment period (n = 8–15/group). B, fat mass expressed as percentage of total body weight at the end of the treatment period (n = 5–9/group). C, basal energy expenditure as measured by 24-h average oxygen consumption (vO₂) (n = 5–9/group). D, intraperitoneal glucose tolerance test. Mice were injected with a bolus of 2 g of glucose/kg body weight after a 16-h fasting period (n = 8–10/group). E, area under curve (in arbitrary units (a.u.)) for the intraperitoneal glucose tolerance test (n = 8–10/group). F and G, blood glucose levels measured in tail vein blood from ad libitum fed mice (n = 8–10/group) (F) or mice fasted for 16 h (n = 8–10/group) (G). Bars, means ± S.E. (error bars). Significant differences (p < 0.05) between CTRL and PGC-1α MKO mice are indicated (#) as well as significant differences between untreated and treated groups (*).