FIGURE 2.

PGC-1α is required for the transcriptional induction of mitochondrial genes in skeletal muscle. A, mRNA levels of PGC-1α/β and mitochondrial genes in SKM relative to Polr2a (RSV-S and RSV-L) or Hprt (SRT) (n = 5–8/group). B, representative immunoblots of mitochondrial proteins in SKM (n = 6/group). C, representative pictures of NADH stainings of SKM sections. Bar graphs show quantification of the intensity of 100 muscle fibers per section (n = 3/group). D, representative electron micrographs from SKM. The bar graph shows average mitochondrial number per image normalized to HFD CTRL and expressed in arbitrary units (a.u.) (n = 3/group). Bars, means ± S.E. (error bars). Significant differences (p < 0.05) between CTRL and PGC-1α MKO mice are indicated (#) as well as significant differences between untreated and treated groups (*). ATP5A, ATP synthase, H⁺-transporting, mitochondrial F1 complex, α subunit 1; UQCRC2, ubiquinol cytochrome c reductase core protein 2; SDHB, succinate dehydrogenase complex, subunit B; eEF2, eukaryotic elongation factor 2.