FIGURE 4.
Functional complementation assay in K616 yeast cells. A, serial dilutions and B Western blotting analysis of yeast K616 cells transformed with pYES2-derived vectors carrying wild-type, D465A mutant, or the C-terminal truncated PMCA3 (PMCAΔ3Cter). Empty vector pYES2 (mock transfected) was used as negative control. C and D, same as A-B but yeast K616 cells are transformed with pYES2-derived vectors carrying wild-type, R482H mutant, or the PMCAΔ3Cter. Total protein lysates from yeast cells carrying the indicated pYES2-derived plasmids were probed with anti-PMCA antibody 5F10. Both monomeric (*) and dimeric (**) PMCA3 forms were detected.