FIGURE 3.

Biochemical properties of the Cav3.2 channel when coexpressed with I-II loop construct. A and B, luminometry detection of the surface expression of the HA-Cav3.2 construct (schematic structure presented in A) in the presence of free GFP (B, white bar), GFP-tagged I-II loop (B, black bar), and GFP-tagged Nter constructs (B, gray bar). C, Western blot analysis of the total HA-Cav3.2 protein amount in cells co-transfected with I-II loop (right lane) compared with the total amount of Cav3.2 protein in cells co-transfected with GFP (middle lane). D, Western blotting (WB) experiments similar to those in C performed in the presence of the Nter construct. E and F, co-immunoprecipitation (IP) experiments for the transfection conditions indicated at the top of the figure showing that the I-II loop protein could be detected (E, arrow) when the HA-Cav3.2 protein was immunoprecipitated but not when the HA-Cav3.2 protein was coexpressed with the Nter (F). Error bars represent S.E.