FIGURE 7.

The thermotolerance of tps1Δ cells can be suppressed by overexpression of HSP genes. A, viability of the CEN.PK tps1Δ strain transformed with a 2μ plasmid bearing HSP104, SSA3, HSP82, or HSC82. Control strains are the wild type TPS1 and tps1Δ cells from the CEN.PK background. Exponentially growing cells on YN Gal medium at 30 °C (open bars) and cells exposed to 42 °C for 2 h (gray bars) are shown. B, viability of the same strains preheated at 37 or 42 °C prior to their transfer to 50 °C for 2 h. “No preheat” condition was control with direct transfer to 50 °C. The data are represented as means ± S.D. of three independent biological replicates.